Journal:

Article Title: High resolution microarray comparative genomic hybridisation analysis using spotted oligonucleotides

doi: 10.1136/jcp.2003.013029

Figure Lengend Snippet: Microarray comparative genomic hybridisation (CGH) profile of the BT474 cell line. (A) Bacterial/phage artificial chromosome polymerase chain reaction representations were used as probe DNAs spotted on to the glass slide; (B) oligonucleotides were used as probe DNAs spotted on to the glass slide. Moving average applied to the log2 ratio of the oligo CGH profile. The vertical bars indicate the spacing between the chromosomes.

Article Snippet: Genomic DNA was isolated from normal kidney (female donor), blood (male donor), or liver (female donor) as a reference, and from the breast cancer cell line BT474, according to Snijders et al . 7 The DNA from the GM00143 cell line (Coriell Institute for Medical Research, Camden, New Jersey, USA) was provided by the Albertson laboratory (University of California, San Francisco, USA).

Techniques: Microarray, Hybridization, Polymerase Chain Reaction

Journal:

Article Title: High resolution microarray comparative genomic hybridisation analysis using spotted oligonucleotides

doi: 10.1136/jcp.2003.013029

Figure Lengend Snippet: Microarray comparative genomic hybridisation (CGH) profile of the long arm of chromosome 17 of the BT474 cell line. Grey triangles, bacterial/phage artificial chromosome polymerase chain reaction representations were used as probe DNAs spotted on to the glass slide; black squares, oligonucleotides were used as probe DNAs spotted on to the glass slide (log2 ratio without moving average). The horizontal bars indicate the two separate amplicons observed.

Article Snippet: Genomic DNA was isolated from normal kidney (female donor), blood (male donor), or liver (female donor) as a reference, and from the breast cancer cell line BT474, according to Snijders et al . 7 The DNA from the GM00143 cell line (Coriell Institute for Medical Research, Camden, New Jersey, USA) was provided by the Albertson laboratory (University of California, San Francisco, USA).

Techniques: Microarray, Hybridization, Polymerase Chain Reaction

Journal: American Journal of Cancer Research

Article Title: KRAS signaling enriched triple negative breast cancer is associated with favorable tumor immune microenvironment and better survival

doi:

Figure Lengend Snippet: KRAS signaling was functioning in the breast cancer cell lines. A. Up-regulation of KRAS expression in breast cancer cell lines when compared with MCF10A. Densitometric values were calculated for KRAS. B. Cell growth suppression after transfection of MB231 cells with MEK inhibitor or PI3K inhibitor at 72 H. Comb: PI3Ki 1 + MEKi 5 (μM). *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Cell culture MCF10A, MCF7, SKBR3, BT474, and MB231 cells were obtained from the JCRB (Japanese Collection of Research Bioresources) Cell Bank.

Techniques: Expressing, Transfection